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. 2020 Dec 10;296:100151. doi: 10.1074/jbc.RA120.016565

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© 2020 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Correlation between the levels of PC(16:0/16:0) and LPC(16:0) in the MCAO brain.A, showing the structures of PC(16:0/16:0) and LPC(16:0). B, a schematic diagram showing membrane-localized PC(16:0/16:0) to LPC(16:0) and the enzyme cPLA2 mediating the conversion of the two in the Lands cycle. Quantifications of PC(16:0/16:0) (C) and LPC(16:0) (D) in the MCAO brain left and right hemispheres (LC and RC, respectively) using the commercial lipid standards as internal controls. The fold changes (RC/LC) of PC(16:0/16:0) and LPC(16:0) were plotted in (E). F, the plot showing the fold changes (RC/LC) of PC(16:0/16:0) and LPC(16:0) from MCAO mice fed with ATK to inhibit cPLA2. Data represent the mean ± SD. Error bars indicate SD. n = 5, ∗ indicates p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by one-way ANOVA with LSD post hoc analysis to identify significant groups when compared between MCAO with the Sham groups.