Figure 5.

LPC(16:0) causes growth cone collapse and neuronal death.A, eGFP transgenic mouse brain cortical explants were cultured for 3 days and treated with the agent as indicated on the microphotograph. Growth cones are indicated by yellow-colored arrows, and collapsed growth cones are shown by the red-colored arrows. The scale bar = 10 μm. B, the % of collapsed growth cones was counted and plotted (n = 10 independent explants per treatment). Neurite length of cortical explants treated with 10 μM of the indicated agent was measured using the Scholl method and plotted in C. The neurite lengths of cortical explants treated with LPC(16:0) (D) and PC(16:0/16:0) (E) at different doses were plotted. Cortical neuronal cultures treated with the indicated LPC(16:0) (F) and PC(16:0/16:0) (G) were examined for cell viability using the CCK-8 kit. DMSO and glutamate treatment were used as negative and positive controls, respectively, for neuronal death. Data represent the mean ± SD. Error bars indicate SD. n = 5 independent repeats, ∗ indicates p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by one-way ANOVA with LSD post hoc analysis to identify significant groups.