Figure 6.

Intracellular calcium influx in neurons in response to LPC(16:0). Cultured cortical neurons were loaded with Fura-2 for calcium imaging.A, treatment with PC(16:0/16:0) at the indicated concentrations did not elicit an increase in [Ca²⁺]i. KCl at 50 mM increased [Ca²⁺]i to show membrane potential changes. In contrast, LPC(16:0) at the indicated concentrations clearly increased [Ca²⁺]i at 10 μM (B), and 50 μM (C). Glutamate receptor inhibitor MK801 failed to block lipid LPC-induced calcium influx. The peak level of [Ca²⁺]i was quantified, as shown in D. Data represents the mean ± SD. Error bars indicate SD. n = 5 independent repeats, ∗∗∗p < 0.001 by one-way ANOVA with LSD post hoc analysis to identify significant groups.