Fig. 4.

Transformation of type O cells from Paramecium decaurelia strain 223 with full-length mtA²²³ results in mating type E expression. Lanes 1–7: seven type O cells from strain 223 were microinjected with a plasmid containing the full-length mtA²²³ gene. Successfully transformed clones were identified by a plasmid-specific PCR and tested for mating types with type O and type E testers from strain 223. The number of + signs below the lanes indicates the strength of the agglutination reaction; – sign, no reaction. Clones 5 and 7 were not sexually reactive and their mating types could therefore not be determined. Lane c1, noninjected control clone (strain 223); lane c2, positive PCR control on the mtA²²³ plasmid.