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. Author manuscript; available in PMC: 2021 Feb 23.
Published in final edited form as: Biochem J. 2017 Feb 20;474(5):781–795. doi: 10.1042/BCJ20160748

Figure 1. Schematic representation of closed (A) and open (B) state Cul-RING Ligase (CRL)s.

Figure 1.

A sketch to present the effect of Cul neddylation in CRLs. Cul have two domains: CulNTD (dark blue) and CulCTD (dark gray) where CulCTD consists of WHB domain, 4HB, and α/β-domains (shown with asterisks). Adaptor and SR proteins bind to CulNTD. Rbx1 (orange) binds to CulCTD. Ubiquitin-carrier E2 enzyme (green) binds to Rbx1 for substrate ubiquitination. Nedd8 (N8, shown in purple) conjugates to the CulCTD H29 of the WHB domain (K724 on Cul5, shown with a pink star) and leads to significant conformational change in CulCTD. Crystal structures report the distance between E2 Cys and the substrate in the closed state as ∼50–60 Å [26,57].