Table 1B.

Immunohistochemical characteristics of 454 colorectal carcinomas with or without CD274 expression

		CD274 expression
	Total No. 454 (100%) [100%]	Positive 54 (12%) [100%]	Negative 400 (88%) [100%]	P-value
MMR status				< 0.001
Deficient	88 (100%) [19%]	40 (45%) [74%]	48 (55%) [12%]
Preserved	366 (100%) [81%]	14 (4%) [26%]	352 (96%) [88%]
CDX2				< 0.001
Negative/weakly-positive	53 (100%) [12%]	29 (55%) [54%]	24 (45%) [6%]
Strongly-positive	401 (100%) [88%]	25 (6%) [46%]	376 (94%) [94%]
ALCAM				< 0.001
Positive	107 (100%) [24%]	26 (24%) [48%]	81 (76%) [20%]
Negative	347 (100%) [77%]	28 (8%) [52%]	319 (92%) [80%]
ALDH1A1				0.52
Positive	132 (100%) [29%]	18 (14%) [33%]	114 (86%) [29%]
Negative	322 (100%) [71%]	36 (11%) [67%]	286 (89%) [72%]
SALL4				1
Positive	22 (100%) [5%]	2 (9%) [5%]	20 (91%) [5%]
Negative	432 (100%) [95%]	52 (12%) [96%]	380 (88%) [95%]
‘Stem-like’ immune phenotype				< 0.001
CDX2-negative/-weak and ALCAM-positive	24 (100%) [5%]	17 (71%) [31%]	7 (29%) [2%]
CDX2-strong and/or ALCAM-negative	428 (100%) [95%]	37 (9%) [69%]	391 (91%) [98%]

P-values were calculated by the χ² test for CD274 expression. The Bonferroni-corrected P-value for significance was P = 0.0041 (0.05/12).