Table 2. Crystallization.

Method	Vapor diffusion, sitting drop
Temperature (K)	290
Protein concentration (mg ml−1)	23
Buffer composition of protein solution	20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
Composition of reservoir solution	10% PEG 8000, 8% ethylene glycol, 100 mM HEPES–NaOH pH 7.5
Protein:precipitant ratio	1:1
Volume of reservoir (µl)	80