Table 1. Macromolecule-production information.
Source organism | S. aureus strain Mu50 |
DNA source | Amplified from S. aureus strain Mu50 genome |
Forward primer† | 5′-CATGCCATGGCAGCTCCACAACAAGGAAC-3′ |
Reverse primer‡ | 5′-CCGCTCGAGTTTCTTTTGGTCGCCATTTGCAG-3′ |
Cloning vector | pET-28a-C6His |
Expression vector | pET-28a-C6His |
Expression host | E. coli Rosetta2 (DE3) |
Complete amino-acid sequence of the construct produced | MAAPQQGTNVNDKVHFTNIDIAIDKGHVNKTTGNTEFWATSSDVLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSKTNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDTYSKDVIVDYGNQKGQQLISSTNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFKIYEVTDQNQFVDSFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQVAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKLEHHHHHH |
The NcoI site is underlined.
The XhoI site is underlined.