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. 2021 Feb 2;77(Pt 2):47–53. doi: 10.1107/S2053230X21000741

Table 1. Macromolecule-production information.

Source organism S. aureus strain Mu50
DNA source Amplified from S. aureus strain Mu50 genome
Forward primer 5′-CATGCCATGGCAGCTCCACAACAAGGAAC-3′
Reverse primer 5′-CCGCTCGAGTTTCTTTTGGTCGCCATTTGCAG-3′
Cloning vector pET-28a-C6His
Expression vector pET-28a-C6His
Expression host E. coli Rosetta2 (DE3)
Complete amino-acid sequence of the construct produced MAAPQQGTNVNDKVHFTNIDIAIDKGHVNKTTGNTEFWATSSDVLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDSKTNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDTYSKDVIVDYGNQKGQQLISSTNYINNEDLSRNMTVYVNQPKKTYTKETFVTNLTGYKFNPDAKNFKIYEVTDQNQFVDSFTPDTSKLKDVTGQFDVIYSNDNKTATVDLLNGQSSSDKQYIIQQVAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKLEHHHHHH

The NcoI site is underlined.

The XhoI site is underlined.