Table 2. Crystallization conditions.
Method | Sitting-drop vapor diffusion |
Plate type | 48-well plates |
Temperature (K) | 277 |
Protein concentration (mg ml−1) | 18 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 8.0, 150 mM NaCl |
Composition of reservoir solution | 0.08 M magnesium formate dihydrate, 25%(w/v) polyethylene glycol 3350 |
Volume ratio of drop | 1:1 |
Volume of reservoir (µl) | 0.5 |