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. 2021 Feb 2;77(Pt 2):47–53. doi: 10.1107/S2053230X21000741

Table 2. Crystallization conditions.

Method Sitting-drop vapor diffusion
Plate type 48-well plates
Temperature (K) 277
Protein concentration (mg ml−1) 18
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 150 mM NaCl
Composition of reservoir solution 0.08 M magnesium formate dihydrate, 25%(w/v) polyethylene glycol 3350
Volume ratio of drop 1:1
Volume of reservoir (µl) 0.5