. 2021 Feb 2;77(Pt 2):37–46. doi: 10.1107/S2053230X21000911

Table 2. Macromolecule-production information for co-purification experiments.

Construct name	Expression vector	Contents
Trx-His₆-hSLAP2	pET32a-mod	Trx, TEV site, thrombin site, SLAP2 29–261
Trx-His₆-Δlinker-hSLAP2	pET32a-mod	Trx, TEV site, thrombin site, SLAP2 29–261
Duet-His-hSLAP2-CBL	pETDuet-1	Trx, TEV site, thrombin site, SLAP2 29–261 in MCS1; CBL 47–357 in MCS2
Duet-His-hSLAP2-CBL 29–261 Δ198–229	pETDuet-1	Trx, TEV site, thrombin site, SLAP2 29–197 and 230–261 in MCS1; CBL 47–357 in MCS2
Duet-His-hSLAP2-CBL 29–254 Δ198–229	pETDuet-1	Trx, TEV site, thrombin site, SLAP2 29–197 and 230–254 in MCS1; CBL 47–357 in MCS2
Duet-His-Δlinker-hSLAP2-CBL 261	pETDuet-1	Trx, TEV site, SLAP2 29–261 in MCS1; CBL 47–357 in MCS2
Duet-His-Δlinker-hSLAP2-CBL 254	pETDuet-1	Trx, TEV site, SLAP2 29–254 in MCS1; CBL 47–357 in MCS2