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. 2020 Oct 19;20(2):e12698. doi: 10.1111/gbb.12698

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© 2020 The Authors. Genes, Brain and Behavior published by International Behavioural and Neural Genetics Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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qRT‐PCR validation of difference in mRNA expression of genes from the alcohol consumption candidate module. The expression levels of three of the transcripts most highly connected to the hub transcript (Lrap) were assessed in brains of alcohol naive male Lrap wild type (^+/+), Lrap heterozygous (^+/−) and Lrap knockout (^−/−) rats (n = 3) by qRT‐PCR. All statistical analyses were done on the delta Ct data and results are reported based on the transformation of mean values and mean values plus one standard error. All p values were calculated using a linear model (Ift81, P2rx4, Txnip) with post hoc pairwise comparisons