TABLE 3.
Possible mediators of the effect of Lrap on alcohol consumption
| Gene symbol | Gene description | Physical location (RN6) | Correlation with alcohol consumption HXB/BXH RI Panel (microarrays) | Correlation with Lrap expression in HXB/BXH RI panel (microarrays) | eQTL in HXB/BXH RI panel (microarrays) | SOI‐level analysis of Lrap−/− vs Lrap+/+ (RNA‐Seq) | SOI‐level analysis of Lrap+/− vs Lrap−/− (RNA‐Seq) | Combination of correlation with Lrap and alcohol consumption in the HXB/BXH and differentiial expression in Lrap−/− and Lrap+/+ | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Correlation Coefficient (p value) | Correlation Coefficient (p value) | Position (95% Bayesian credible interval) | Genome‐wide p value | Ratio of KO to WT | Unadjusted p value | Ratio of HET to WT | Unadjusted p value | p Value | FDR | |||
| Cndp1 | carnosine dipeptidase 1 | Chr18: 81.5 Mb | 0.63 (0.002) | −0.51 (0.0193) | Chr6: 6.6 Mb [3.6 to 145.8] | 0.21 | 1.77 | 0.036 | 1.38 | 0.032 | 1.7E‐06 | 9.5E‐04 |
| Ninj2 | ninjurin 2 | Chr4: 152.6 Mb | 0.53 (0.012) | −0.63 (0.0024) | Chr4: 166.1 Mb [136.6 to 166.1] | 0.025 | 1.3 | 0.045 | 1.06 | 0.411 | 1.3E‐06 | 9.5E‐04 |
| P2rx4 | purinergic receptor P2X 4 | Chr12: 39.3 Mb | −0.63 (0.002) | 0.84 (<0.0001) | Chr12: 39.1 Mb [39.1 to 41.5] | 0.034 | 0.7 | 0.006 | 0.67 | 0.003 | 3.3E‐11 | 2.9E‐07 |
| Slc35c2 | solute carrier family 35 member C2 | Chr3: 161.8 Mb | −0.57 (0.008) | 0.53 (0.0142) | Chr17: 61.9 Mb [2.3 to 89.6] | 0.375 | 1.47 | 0.001 | 1.44 | 0.002 | 1.4E‐07 | 2.4E‐04 |
| Zfr2 | zinc finger RNA binding protein 2 | Chr7: 11.3 Mb | 0.46 (0.035) | −0.46 (0.0364) | Chr9: 117.7 Mb [15.3 to 120] | 0.232 | 1.38 | 0.028 | 1.14 | 0.229 | 3.6E‐05 | 4.2E‐03 |
| Znhit6 | zinc finger, HIT‐type containing 6 | Chr2: 251.5 Mb | −0.48 (0.028) | 0.51 (0.0174) | Chr2: 251 Mb [146.8 to 259.9] | 0.409 | 0.64 | 0.009 | 0.92 | 0.972 | 4.3E‐06 | 1.7E‐03 |
Note: Sums of isoforms (SOI) are included if they were correlated with alcohol consumption in the HXB/BXH recombinant inbred rat panel using the microarray data (unadjusted p value<0.05), correlated with Lrap in the HXB/BXH RI panel (unadjusted p value<0.05), and differentially expressed between the Lrap−/− and the wild type Lrap+/+ rat in the RNASeq study (unadjusted p value<0.05).