FIGURE 8.

Comparison of mRNA transfection with DOTAP and several HK peptides. To form lipopolyplexes, the HK peptides (4 μg) in Opti‐MEM were initially mixed with mRNA (1 μg) for 30 minutes. DOTAP liposomes (1 μg) were then added, gently mixed and allowed to stand for an additional 30 min. Polyplexes were prepared as described previously. These complexes in Opti‐MEM (50 μl) were then added to the cells and, 24 hours later, the luciferase activity was measured. *p < 0.05; **p < 0.01; ***p < 0.001