FIGURE 2.

Verification of JEVpr4/DENV2 chimeric virus and production of chimeric virus antibody. (A) Indirect immunofluorescence detection of JEVpr4/DENV2 infectivity to BHK21 and C6/36 cells. (B) Plaque morphology of DENV2 in C6/36 plaque. The plaques formed by the JEVpr4/DENV2 chimeric virus were smaller than DENV2‐ZS01/01. (C) The titers of JEVpr4/DENV2 and DENV2‐ZS01/01 at different time points in C6/36 cells. The replication of the JEVpr4/DENV2 chimeric virus in C6/36 cells was significantly lower than that of DENV2‐ZS01/01, where the highest titers appeared 48 hours after infection for JEVpr4/DENV2, whereas DENV2‐ZS01/01 took only 24 hours