FIGURE 7.

Bioinformatics analysis of key amino acid sites. (A) Alignment of amino acids of the pr protein of flaviviruses with DENV1–4. Amino acids 5, 6, and 7 of the pr4 peptide are conserved among different DENV serotypes, which are different from other members of the genus Flavivirus. However, cysteine at position 16 is conserved among members of the genus Flavivirus. (B) The tertiary structure of pr peptide was obtained by Swiss‐Model homology modeling. Amino acids 5, 6, 7 and 16 of pr4 formed a pocket‐like structure, which increased the possibility for these four sites being the key amino acid sites in antibody‐binding epitopes