Figure 3.

Association of cAMP‐response element binding protein (CREB)‐regulated transcription coactivator (CRTC)2 and CRTC3 at the Star promoter. Binding of CRTC2, CRTC3 and non‐specific rabbit immunoglobulin (Ig)G at the Star promoter and at Star exon 5 of the Star gene in the rat adrenal following (A) injection with 10 ng of adrenocorticotrophic hormone (ACTH), mimicking an ultradian ACTH pulse (n = 3‐4 independent rats per group) or (B) following endotoxic stress by injection with 25 μg of lipopolysaccahride (n = 4‐6 independent rats per group). Binding of these proteins was measured by a chromatin immunoprecipitation assay and quantified as the percentage pulldown of chromatin input. Data are represented as the mean ± SEM and analysed by two‐way one‐way analysis of variance (ANOVA) followed by Tukey’s post‐hoc test. *P ≤ 0.05, ***P ≤ 0.001 vs 0 min. ##P ≤ 0.01, ###P ≤ 0.001 vs maximum levels