Figure 4.

Variation in cAMP‐response element binding protein (CREB)‐regulated transcription coactivator (CRTC)1‐3 expression across 24 hours. (A) Plasma adrenocorticotrophic hormone (ACTH) (pg mL^‐1), (B) plasma corticosterone (ng mL^‐1) and (C) a representative western immunoblot of whole cell CRTC1, CRTC2 and CRTC3 protein levels and semi‐quantification of CRTC1, CRTC2 and CRTC3, normalised to α‐tubulin (P = 0.782 by one‐way one‐way analysis of variance [ANOVA]) and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (P = 0.142 by one‐way ANOVA). For visualisation purposes, the value at 5.00 am has been plotted twice. The grey bar indicates the period of lights off (7.00 pm to 5.00 am). Data are represented as the mean ± SEM (n = 5 or 6 per time point); data in (C) are expressed as fold induction of levels at 5 am. ACTH and CRTC1, CRTC2 and CRTC3 protein data were analysed using one‐way ANOVA followed by Tukey’s post‐hoc test. Corticosterone data were analysed using Welch’s ANOVA followed by Games‐Howell post‐hoc test. *P ≤ 0.05 vs 5 am