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. Author manuscript; available in PMC: 2021 Feb 23.
Published in final edited form as: J Leukoc Biol. 2019 Jun 19;106(4):967–975. doi: 10.1002/JLB.3AB0518-180RR

Figure 3.

Figure 3.

IFNAR blockade reduces IL-10 and CD40, and CXCL10 in TCSle cDCs response to CpG and R848 stimulation. (a-f) cDCs were treated with anti-IFNAR antibody or mIgG isotype control for 1 hour prior to stimulation with R848 (1 μg/ml) and CpG (10 μg/ml) for 24 hours. (a-b) Supernatants were collected 24 hours after stimulation and IL-10 was measured by ELISA. (c-d) Supernatants were collected 24 hours after stimulation and CXCL10 was measured by ELISA. (e-f) cDCs were collected at 24 hours after stimulation and stained. Samples were gated on singlets, scatter gate, live cells, CD11c+ CD11b+, CD40+. Dotted lines represent PBS treated B6 (20.6%) or TCSle (22.7%) CD40 expression (a-f) Averages and SE of 4–6 replicates from 3 experiments. (a-b) Due to inter-experimental variation, B6 stimulation condition is set to 1. Statistical significance was calculated by ANOVA and post-hoc Tukey’s multiple comparison test with the exception of (a-b) gray stars representing statistical significance calculated by unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001.