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Chemical shift differences between m7GpppApG and m7GTP bound eIF4E color coded on the protein surface (PDB id: 1L8B) and fragments of 15N HSQC spectra of eIF4E/m7GpppApG (pink contours) and eIF4E/m7GTP (blue contours) presenting selected chemical shift perturbations (CSPs calculated as sqrt[ΔδH2 + (0.15·ΔδN)2]. A fragment of C-terminal loop that was not assigned in the spectra is colored red. Possible RNA path deduced from CSPs is indicated by a yellow dotted line.
