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. Author manuscript; available in PMC: 2021 Feb 23.
Published in final edited form as: Nat Genet. 2020 Mar 16;52(4):418–427. doi: 10.1038/s41588-020-0591-8

Extended Data Fig. 4. Deficiency in activation of endogenous FOXA1 liver target genes by FOXA-ΔHx and FOXA1-PP mutant proteins.

Extended Data Fig. 4

a, Schematic of functional assay for FOXA1 wild-type and mutants in H2.35 liver cells. b, Apoa1 and Ttr1 expression analysis by RT-qPCR relative to expression levels in the control. Results shown as mean +/− s.e.m. of four biological replicates. P values are from two-sided Student's t-test. c, Western blot analysis of two biological replicates for endogenous and exogenous FOXA proteins, demonstrating similar amounts of FOXA-ΔHx and FOXA1-PP mutant proteins as FOXA1-WT, and thus indicating an intrinsic deficiency in the mutants' abilities to restore expression of endogenous liver genes in a Foxa1 knock-down background. Two experiments were repeated independently with similar results. Full blots are presented in the Source Data files.