Fig. 2.

Effect of the quantity of DiI on ND size and size distribution. NDs were sonicated for a total active time of 60 s at 72 W, with a pulse of 7 s and 30% duty cycle. A) The boxplots show the diameter of NDs produced with different amounts of DiI. Increasing the quantity of DiI led to a small decrease in the size of NDs from 240.5 ± 23.7 to 211.8 ± 16.7 nm (*p < 0.05) B) Boxplots showing the polydispersity index (PDI) of NDs produced with different amounts of DiI. Three different samples were tested for each case (N = 3). C) The microscope image (40x oil immersion) shows three MG63 cells, where the blue circle is the cell nucleus stained with DAPI dye, while the yellow spots are NDs stained with DiI. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)