Fig. 2.

Suppression of circAGAP1 expression reduced the proliferation, migration and invasion of ccRCC cells. a, b qRT-PCR analysis of circAGAP1 in A498 and ACHN cells transfected with si-circAGAP1 or si-NC. Data are presented as the means ± SEM from three independent experiments. c, d The viability of A498 and ACHN cells transfected with si-circAGAP1 or si-NC was determined by the CCK-8 assay. e EdU analysis and quantification of EdU-positive A498 and ACHN cells transfected with si-circAGAP1 or si-NC. f Apoptotic rates of A498 and ACHN cells transfected with si-circAGAP1 or si-NC as detected by flow cytometry. g, h Transwell assays of A498 and ACHN cells transfected with si-circAGAP1 or si-NC. The experiments were repeated with at least three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001