Fig. 4.

circAGAP1 sponged miR-15a-5p. a The interaction between miR-15a-5p and circAGAP1 was assessed by biotin-coupled probe pull-down assay. Data are presented as the means ± SEM from three independent experiments. b Detection of colocalization of miR-15a-5p and circAGAP1 in A498 and ACHN cells by RNA-FISH assay. Nuclei were stained with DAPI (magnification, × 400). Red, miR-15a-5p; Green, circAGAP1; Blue, DAPI. c Wild-type (WT) and associated mutant (MUT) sequences of the potential miR-15a-5p binding sites in circAGAP1. The effect of the miR-15a-5p mimic on the luciferase activities of WT or MUT circAGAP1 was detected. d Expression of miR-15a-5p in ccRCC and adjacent noncancerous tissues was analyzed (N = 25, p = 0.0008). The experiments were repeated with at least three biological replicates. **p < 0.01, ***p < 0.001, ****p < 0.0001