Fig. 5.

miR-15a-5p partially reversed the circAGAP1-induced proliferation, migration and invasion effects in ccRCC. a EdU analysis and quantification of EdU-positive A498 and ACHN cells transfected with mock, circAGAP1, circAGAP1 + miR-NC or circAGAP1 + miR-15a-5p mimics. Cells were subjected to hypoxia for 24 h. b Apoptotic rates of A498 and ACHN cells transfected with mock, circAGAP1, circAGAP1 + miR-NC or circAGAP1 + miR-15a-5p mimics as assessed by flow cytometry. Cells were subjected to hypoxia for 24 h. c, d Transwell assays of A498 and ACHN cells transfected with mock, circAGAP1, circAGAP1 + miR-NC or circAGAP1 + miR-15a-5p mimics. The experiments were repeated with at least three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001