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. 2021 Feb 23;40:77. doi: 10.1186/s13046-021-01870-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Claudin-2 knockdown in PTE cells increases plasticity and proliferation: MDCKII^TALEN cells where claudin-2 expression was knocked down by TALEN mediated genetic editing were subjected to the EMT analysis compared to the control MDCKII cells. a-b Immunoblot and its quantitation analysis using total cell lysates from MDCK-II and MDCKII^TALEN cells; (c-d) Immunoblotting and densitometric analysis using total cell lysates from MDCK-II and MDCKII^TALEN cells subjected to the EGF (100 ng/ml) treatment in time dependent manner; (e) Quantitative analysis of Ki-67 positive cells in MDCKII and MDCKII^TALEN cells treated with or without EGF; (f) Quantitative analysis of the wound-healing in MDCKII and MDCKII^TALEN cells; (g) Cell cycle analysis of MDCKII and MDCKII^TALEN cells by flow cytometry using PI staining. h The quantitative measurement of different cell cycle phases; (i-j) Representative immunoblots and quantitation of EMT related and claudin proteins in control HK-2 cells (CON^siRNA) vs cells expressing anti-human claudin-2 siRNA (Cldn2^siRNA). Data is presented as mean + sem. Statistical significance was determined by student t test and 1-way ANOVA and post hoc Tukey’s test for pairwise comparison. ** P < 0.01, ***P < 0.001