Table 8.
Phenotypic analysis of progenies of plants regenerated from Ws-2 protoplasts
| Wild-type | R1 #1 | R1 #2 | R1 #3 | |
|---|---|---|---|---|
| Seed germination (%) | 98.04 ± 0.80 | 97.06 ± 1.39 | 100 | 99.02 ± 0.80 |
| Cotyledon area (mm2) | 4.84 ± 0.15 | 4.88 ± 0.22 | 5.16 ± 0.22 | 4.93 ± 0.21 |
| Flowering time (Rosette leaf no.) | 8.37 ± 0.75 | 8.62 ± 0.85 | 8.27 ± 0.70 | 8.69 ± 0.67 |
Harvested R1 seeds from protoplast-regenerated plants were germinated on MS medium (> 60 seeds). Germination rate was calculated as the percentage of radicle emergence from the seed coat at 2 days after transfer to the long-day condition. Cotyledon area of 7-day-old seedlings was measured with ImageJ software. Flowering time was assessed as the number of rosette leaves, when the bolt reached 1 cm. Data represent the mean ± SEM of biological triplicates