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. 2021 Feb 23;34(10):108837. doi: 10.1016/j.celrep.2021.108837
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat anti Monkey IgG (HRP) antibody Fitzgerald Industries International Cat# 43R-IG020HRP, RRID:AB_1287018 Lot# c15032720
SARS Nucleocapsid Protein Antibody Novus Biologicals Cat# NB100-56683, RRID:AB_838841 Lot # 111003D-4
Biotinylated Goat Anti-Rabbit IgG antibody Vector Laboratories Cat# BA-1000, RRID:AB_2313606 Lot # ZG0122
Anti-fibrin monoclonal mouse primary antibody Sekisui Diagnostics Cat# REF 350 Lot# 140714
Goat Anti-Mouse IgG Antibody (H+L), Biotinylated Vector Laboratories Cat# BA-9200, RRID:AB_2336171 Lot # ZB0324

Bacterial and virus strains

SARS-CoV-2 European Virus Archive SARS-CoV-2/INMI1-Isolate/2020/Italy

Biological samples

Pooled Convalescent Sera (“High Dose”) Woolsey et al., 2021 N/A
Pooled Convalescent Sera (“Low Dose”) Cross et al., 2020 N/A

Chemicals, peptides, and recombinant proteins

RT SigmaFast O-phenylenediamine (OPD) substrate Sigma-Aldrich Cat# P9187
Alkaline Phosphatase Streptavidin Vector Laboratories Cat# SA-5100-1
Bio-Red developer Bio-Path Cat# BP-100-FR

Critical commercial assays

ReSARS® CoV-2 (N) IgG ELISA Kit – RUO Zalgen Labs, LLC Cat# 10166
ReSARS® CoV-2 (S-RBD) IgG ELISA Kit – RUO Zalgen Labs, LLC Cat# 10180-1
QIAamp Viral RNA Mini Kit QIAGEN Cat# 52906
QuantiFast Probe RT-PCR Kit QIAGEN Cat# 204454
One-Step Trichrome Blue & Red Stain Kit American Mastertech Cat# ML7229
Legendplex Multiplex Assay: Human Thrombosis Panel BioLegend Cat# 740891
Legendplex Multiplex Assay: NHP Inflammation Panel BioLegend Cat# 740389
Piccolo Biochemistry Panel Plus analyzer discs Abaxis Cat# 400-7182-1
Vetscan VSPro PT/aPTT Combination test Abaxis Cat# 740-7013
Vetscan VSPro Fibrinogen test Abaxis Cat# 740-7031

Experimental models: cell lines

Vero E6 ATCC ATCC CRL-1586

Experimental models: organisms/strains

African green monkey Worldwide Primates, Inc. N/A

Oligonucleotides

2019-nCoV_N2 Forward Primer: TTA CAA ACA TTG GCC GCA AA U.S. Centers for Disease Control and Prevention https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html
2019-nCoV_N2 Reverse Primer: GCG CGA CAT TCC GAA GAA U.S. Centers for Disease Control and Prevention https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html
2019-nCoV_N2 Probe: FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 U.S. Centers for Disease Control and Prevention https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html

Software and algorithms

Biotek Cytation5 v.3.04 BioTek (Agilent) Packaged with Instrument
CFX Maestro 1.1 version v4.1.2433.1219 Bio-Rad Packaged with Instrument
LEGENDplex Data Analysis Software BioLegend https://www.biolegend.com/en-us/legendplex
BD FACS Diva BD Biosciences Packaged with Instrument
GraphPad v.9.0.0 GraphPad https://www.graphpad.com/scientific-software/prism/