Antibodies |
|
Goat anti Monkey IgG (HRP) antibody |
Fitzgerald Industries International |
Cat# 43R-IG020HRP, RRID:AB_1287018 Lot# c15032720 |
SARS Nucleocapsid Protein Antibody |
Novus Biologicals |
Cat# NB100-56683, RRID:AB_838841 Lot # 111003D-4 |
Biotinylated Goat Anti-Rabbit IgG antibody |
Vector Laboratories |
Cat# BA-1000, RRID:AB_2313606 Lot # ZG0122 |
Anti-fibrin monoclonal mouse primary antibody |
Sekisui Diagnostics |
Cat# REF 350 Lot# 140714 |
Goat Anti-Mouse IgG Antibody (H+L), Biotinylated |
Vector Laboratories |
Cat# BA-9200, RRID:AB_2336171 Lot # ZB0324 |
|
Bacterial and virus strains |
|
SARS-CoV-2 |
European Virus Archive |
SARS-CoV-2/INMI1-Isolate/2020/Italy |
|
Biological samples |
|
Pooled Convalescent Sera (“High Dose”) |
Woolsey et al., 2021 |
N/A |
Pooled Convalescent Sera (“Low Dose”) |
Cross et al., 2020 |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
RT SigmaFast O-phenylenediamine (OPD) substrate |
Sigma-Aldrich |
Cat# P9187 |
Alkaline Phosphatase Streptavidin |
Vector Laboratories |
Cat# SA-5100-1 |
Bio-Red developer |
Bio-Path |
Cat# BP-100-FR |
|
Critical commercial assays |
|
ReSARS® CoV-2 (N) IgG ELISA Kit – RUO |
Zalgen Labs, LLC |
Cat# 10166 |
ReSARS® CoV-2 (S-RBD) IgG ELISA Kit – RUO |
Zalgen Labs, LLC |
Cat# 10180-1 |
QIAamp Viral RNA Mini Kit |
QIAGEN |
Cat# 52906 |
QuantiFast Probe RT-PCR Kit |
QIAGEN |
Cat# 204454 |
One-Step Trichrome Blue & Red Stain Kit |
American Mastertech |
Cat# ML7229 |
Legendplex Multiplex Assay: Human Thrombosis Panel |
BioLegend |
Cat# 740891 |
Legendplex Multiplex Assay: NHP Inflammation Panel |
BioLegend |
Cat# 740389 |
Piccolo Biochemistry Panel Plus analyzer discs |
Abaxis |
Cat# 400-7182-1 |
Vetscan VSPro PT/aPTT Combination test |
Abaxis |
Cat# 740-7013 |
Vetscan VSPro Fibrinogen test |
Abaxis |
Cat# 740-7031 |
|
Experimental models: cell lines |
|
Vero E6 |
ATCC |
ATCC CRL-1586 |
|
Experimental models: organisms/strains |
|
African green monkey |
Worldwide Primates, Inc. |
N/A |
|
Oligonucleotides |
|
2019-nCoV_N2 Forward Primer: TTA CAA ACA TTG GCC GCA AA |
U.S. Centers for Disease Control and Prevention |
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html |
2019-nCoV_N2 Reverse Primer: GCG CGA CAT TCC GAA GAA |
U.S. Centers for Disease Control and Prevention |
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html |
2019-nCoV_N2 Probe: FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 |
U.S. Centers for Disease Control and Prevention |
https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html |
|
Software and algorithms |
|
Biotek Cytation5 v.3.04 |
BioTek (Agilent) |
Packaged with Instrument |
CFX Maestro 1.1 version v4.1.2433.1219 |
Bio-Rad |
Packaged with Instrument |
LEGENDplex Data Analysis Software |
BioLegend |
https://www.biolegend.com/en-us/legendplex |
BD FACS Diva |
BD Biosciences |
Packaged with Instrument |
GraphPad v.9.0.0 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/ |