Figure S4.

No improvement of viral replication of selection IV ΔORF3-E (S-IV-P5) mNG virion after 10 rounds of culturing on Vero E6 cells, related to Figure 2

S-IV-P5 mNG virion was continuously passaged on Vero E6 cells for 10 rounds. The resulting P2 and P10 S-IV-P5 mNG virions (i.e., S-IV-P5-Vero-P2 and S-IV-P5-Vero-P10, respectively) were used to infect Vero E6 cells at an MOI of 0.001. The mNG-positive cells (A) and the growth kinetics of the S-IV-P5-Vero-P2 and S-IV-P5-Vero-P10 virions (B) were compared. Scale bar, 100 μm. We did not use the S-IV-P5-Vero-P1 virion in this experiment because the P1 stock retained some carryover virions derived from the Vero-ORF3-E trans-complementation culture. Viral titers were analyzed by unpaired t test. ns, p > 0.05. Data are represented as mean ± SD.