Fig. 5.

Multimodal CARS/SHF/TPEF bioimaging with our new OPA/SHG source. Images are of mouse adipose tissue acquired at the surface of the sample. (a) Schematic showing the main components of adipose tissue; (b) CARS image mapping the intensity of the 2845 ${\mathrm{cm}}^{-1}$ lipid band across the tissue; (c) collagen fibers imaged with SHG; (d) TPEF images FAD autofluorescence in macrophage cells; (e) composite image with CARS, SHG and TPEF overlaid. Color intensity relates linearly to the counts on the detector, normalized to the maximum to obtain the highest contrast for each of the channels independently. The images shown are single frame CARS acquisitions with pixel dwell time of 8 µs, 28 mW pump and 30 mW of Stokes.