KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PEcy7 anti-mouse Tnf-alpha	ThermoFisher	Cat# 25–7321-82; RRID:AB_11042728
Isotype control	Biolegend	Cat#400416; RRID:AB_326522
Fc block	BD PharMingen	Cat#553142; RRID:AB_394657
Chemicals, Peptides, and Recombinant Proteins
E.coli LPS	InvivoGen	Cat#tlrl-3pelps
Lipofectamine 2000	ThermoFisher	Cat#11668019
iScript Reverse Transcription Supermix	Biorad	Cat#1708841
iQ SYBR Green Supermix	Biorad	Cat#1725122
Critical Commercial Assays
Mouse TNF-alpha DuoSet ELISA	R&D Systems	Cat#DY410
Direct-zol RNA MiniPrep Kit	Zymo	Cat#R2072
TruSeq Stranded Total RNA Library Prep kit	Illumina	Cat#20020597
Deposited Data
Raw and analyzed data	This paper	GEO: GSE138786
Raw and analyzed data	This paper	GEO: GSE138788
Experimental Models: Cell Lines
immortalized bone-marrow-derived macrophages with an NFKB reporter and Cas9 (iBMDM-NFKB-Cas9)	Covarrubias et al., 2017	PMID: 29051223
THP-1 cells	ATCC	ATCC TIB202
Oligonucleotides
sgRNA sequences, see Table S1	This paper	N/A
Custom sequencing primer:	This paper	N/A
5′GAGACTATAAGTATCCCTTGGAGAACCACCTTGTTGG-3′
Il6 qRT-PCR primer F: AACGATGATGCACTTGCAGA	IDT	N/A
Il6 qRT-PCR primer R: GAGCATTGGAAATTGGGGTA	IDT	N/A
Gapdh qRT-PCR primer F: CCAATGTGTCCGTCGTGGATCT	IDT	N/A
Gapdh qRT-PCR primer R: GTTGAAGTCGCAGGAGACAACC	IDT	N/A
Hprt qRT-PCR primer F: TGCTCGAGATGTCATGAAGG	IDT	N/A
Hprt qRT-PCR primer R: TATGTCCCCCGTTGACTGAT	IDT	N/A
Recombinant DNA
Plasmid: psPAX2	Addgene	Addgene Plasmid #12260; RRID:Addgene_12260
Plasmid: pMD2.G	Addgene	Addgene Plasmid #12259; RRID:Addgene_12259
Software and Algorithms
FlowJo	BD	https://www.flowjo.com/
MAGeCK	Li et al., 2014	PMID: 25476604
gRNA_Tool		https://github.com/quasiben/gRNA_Tool
Mann-Whitney U test	Gilbert et al., 2014	PMID: 25307932
TopHat	Kim et al., 2013	PMID: 23618408
DESeq2 R package	Love et al., 2014	PMID: 25516281