Figure 1.

EGF mediated down regulation of UGT2B15, UGT2B17, DNMT3A and DNMT3B expression is reversed by EGFR inhibitor, PD168393 (PD). LNCaP cells were treated for 24 h with vehicle (DMSO), EGF and EGF+PD at concentrations indicated in the figure. mRNA was extracted and quantitated using RT-PCR as described in materials and methods. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. The mRNA levels are reported as the mean ± SEM of 3 independent experiments, each performed in triplicate. Means that do not share letters are significantly different from each other. (a) Effect of EGF and PD168393 on UGT2B15 expression. (b) Effect of EGF and PD168393 on UGT2B17 expression (c) Effect of EGF and PD168393 on DNMT3A expression. (d) Effect of EGF and PD168393 on DNMT3B expression. Vehicle = Control