Figure 4.

DNA methyltransferase inhibitor RG108 treatment of LNCaP cells downregulates UGT2B17 expression. LNCaP cells were treated with vehicle (DMSO) and RG108 (100 µM) for 48 h. mRNA was quantitated as described in material and methods. Data was analysed using Nonparametric Student t test. mRNA levels are reported as the mean ± SEM of experiments done in triplicate. (a) Effect of RG108 treatment on DNMT3A expression. (b) Effect of RG108 treatment on DNMT3B expression. (c) Effect of RG108 treatment on UGT2B17 expression . (d) Effect of RG108 on p53 expression. Vehicle = Control