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Primers used for sgRNA cloning
| Targeting site | Sequences of primers (5ʹ-3ʹ) |
|---|---|
| egfp | F: CACCGGGGCGAGGAGCTGTTCACCG |
| R: AAACCGGTGAACAGCTCCTCGCCCC | |
| fiber-2 | F: CACCGCGTGCTCTACAGCTGTCCAG |
| R: AAACCTGGACAGCTGTAGAGCACGC | |
| KPNA3 | F:CACCGATGGCCGAGAACGCCGCCGC |
| R: AAACGCGGCGGCGTTCTCGGCCATC | |
| KPNA4 | F: CACCGGTTGTCCAGTTTCTCGTTGT |
| R: AAACACAACGAGAAACTGGACAACC |
