Figure 2.

Unique characteristics of accessible chromatin in bovine oocytes and early embryos. (a) The enrichment of ATAC-seq peaks at annotated promoters (TSS ± 5kb) and annotated transcription end sites (TES ± 5kb) (normalized and on average) in oocytes and embryos. The enrichment was measured at individual base pair level by FPM (Fragments per Million mapped reads). (b) The genome browser view showing the enrichment of ATAC-seq peaks near two active genes (GATA3, left panel; FOXA1, right panel) in oocytes and embryos. ATAC-seq peaks near TSSs and TESs were shaded in different colours (TSSs in yellow; TESs in green). (c) Enrichment of ATAC-seq peaks in the promoters and distal peaks (right panel), and repetitive elements (left panel). (d) Transcription factor motifs identified from distal ATAC-seq peaks at each stage during early embryo development