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. 2020 Mar 11;63(7):3701–3712. doi: 10.1021/acs.jmedchem.0c00012

Figure 2.

Figure 2

PXR and CYP3A4 expression in HepG2 cells treated with garcinoic acid (1). siRNA technique was used to transiently inhibit PXR (A) or CYP3A4 (B) gene expression [*p < 0.05; **p < 0.01 vs WT or Ctr test; #p < 0.01 vs Rifampicin (RIF)]. PXR (C) and CYP3A4 (D) protein and mRNA (E) expression were also assessed in cells treated for 24 h with 1 and 25 μM GA (1). MDR1 mRNA expression (F) was evaluated at concentrations between 1 and 50 μM GA. The P-glycoprotein activity (G) was measured in the presence of GA between 50 nM and 25 μM (●), and Verapamil (▲) was used as a control. t test: control versus treatments, *p < 0.05; **p < 0.01.