PXR, CYP3A4, and MDR1 expression in the intestine of mice treated with garcinoic
acid (1). Mice were treated with increasing doses of GA from 5 to 25 mg
administered as a single bolus, and PXR mRNA (A) and protein expression (B, left
panel) were measured in liver samples 24 h post-treatment. CYP3A4 (CYP3A11 in mice)
and MDR1 protein expression were also investigated (B, middle and right panels);
*p < 0.05; **p < 0.01. Liver histology was
examined by hematoxylin and eosin (H&E) staining and IHC for PXR antigen
(C).