FIGURE 1.

IL‐22 preserves mitochondrial fitness and glycolysis in TECs. (A and B) We use Seahorse XF96 Extracellular Flux Analyzer to test the changes in the extracellular acid rate (ECAR) and oxygen consumption rate (OCR) of TECs. OCR and ECAR in TECs incubated with 50 mM glucose, or 5 µg/mL cisplatin, or 0.2 mM palmitic acid, or 4 µM DOX in the presence or absence of IL‐22. (C) Maximal respiratory capacity (MRC) of TECs assessed by real‐time changes in OCR. (D and E) Curves in the OCR and ECAR of TECs after incubated with oligomycin, FCCP, glucose, rotenone, and 2‐DG. (F) Localization and expression of nuclear (blue), Glut1 (green), and plasma membrane (red) in TECs. (G) Glucose consumption in TECs after IL‐22 treatment for 24 h. n = 3; scale bars, 20 µm; Student's t‐test (unpaired); ***p < 0.001, **p < 0.01, *p < 0.05