FIGURE 3.

Induction of AMPK/AKT signaling by IL‐22 maintains mitochondrial fitness. TECs were stimulated 50 mM glucose, or 5 µg/mL cisplatin, or 0.2 mM palmitic acid, or 4 µM DOX in the presence or absence of IL‐22, Compound C, or LY294002 for indicated times. (A and B) AMPK/AKT signaling pathway activation in TECs or STAT3‐WT and STAT3–KD TECs was evaluated by western blot analysis. (C and D) Changes in the OCR and ECAR of TECs were analyzed. (E) AMPK/AKT signaling pathway activation in TECs was evaluated by western blot analysis. (F–I) Changes in the OCR and ECAR of TECs were measured in TECs versus Compound C and LY294002 treated TECs. Student's t‐test (unpaired); n = 3; ***p < 0.001, **p < 0.01, *p < 0.05