Fig 9. qPCR validation of RNA-seq results.

Quantitative reverse transcription PCR (RT-qPCR) validation of four immune genes (IFN-γ, TNF-α, IL-10, IL-1β) found to be significantly differentially expression by RNA-seq at day 7 in the gills. The X-axis shows the gene and phenotype assayed and the Y-axis shows the relative log₂(Fold Change) between fish exposed to Ceratonova shasta and their respective control. Error bars indicate the standard deviation of Cq values between biological replicates.