Table 3. Comparison of Abbott m2000 SARS-CoV-2 PCR test and DiaCarta QuantiVirus™ SARS-CoV-2 PCR test for SARS-CoV-2 detection in clinical saliva samples.
Method Comparison | Abbott m2000 Real-time SARS-CoV-2 | Diacarta QuantiVirus SARS-CoV-2 multiplex | |||||
---|---|---|---|---|---|---|---|
Accession # | Detection & qPCR Ct | Detection | ROX(E-Gene) | Cy5(N-Gene) | FAM(ORF 1ab gene) | VIC(RP Gene) | |
Patient 1 | Saliva 1 | Not Detected | Not Detected | Undetermined | Undetermined | Undetermined | 21.6 |
Patient 2 | Saliva 2 | Not Detected | Not Detected | Undetermined | undetermined | Undetermined | 22.9 |
Patient 3 | Saliva 3 | Not Detected | Not Detected | Undetermined | undetermined | Undetermined | 22.1 |
Patient 4 | Saliva 4 | Not Detected | Not Detected | Undetermined | undetermined | Undetermined | 21.6 |
Patient 5 | Saliva 5 | Detected (Ct 18.21) | Detected | 32.1 | 31.7 | 30.0 | 20.7 |
Patient 6 | Saliva 6 | Detected (Ct 31.00) | Detected | Undetermined | 37.8 | Undetermined | 23.7 |
Patient 7 | Saliva 7 | Not Detected | Not Detected | Undetermined | Undetermined | Undetermined | 19.7 |
Patient 8 | Saliva 8 | Not Detected | Detected | 37.4 | 37.3 | 42.4 | 23.3 |
Patient 9 | Saliva 9 | Detected (Ct 23.89) | Detected | Undetermined | 24.4 | undetermined | 21.8 |
Patient 10 | Saliva 10 | Not Detected | Not Detected | Undetermined | undetermined | Undetermined | 22.3 |
Patient 11 | Saliva 11 | not detected | Detected | 32.8 | 33.9 | 31.5 | 23.9 |
Patient 12 | Saliva 12 | not detected | Detected | 35.7 | 38.5 | 33.9 | 24.2 |
Patient 13 | Saliva 13 | Not Detected | Not detected | Undetermined | 43.5 | Undetermined | 24.5 |
Patient 14 | Saliva 14 | Detected (Ct 20.77) | Detected | 36.1 | 37.0 | 34.0 | 26.1 |
Patient 15 | Saliva 15 | Detected | Detected | 35.6 | 35.1 | 34.2 | 23.6 |
Patient 16 | Saliva 16 | Not Detected | Not detected | Undetermined | 37.7 | Undetermined | 23.6 |
Patient 17 | Saliva 17 | Not Detected | Not detected | Undetermined | Undetermined | 41.2 | 32.7 |
Patient 18 | Saliva 18 | Not Detected | Not detected | Undetermined | Undetermined | Undetermined | 24.9 |
Patient 19 | Saliva 19 | Not Detected | Not detected | Undetermined | Undetermined | 33.5 | 29.1 |
Patient 20 | Saliva 20 | Detected (Ct. 21.40) | Detected | Undetermined | 39.4 | 36.8 | 26.6 |
Patient 21 | Saliva 21 | Not Detected | Not detected | Undetermined | Undetermined | Undetermined | 25.1 |
Patient 22 | Saliva 22 | Not Detected | Not detected | Undetermined | 43.5 | Undetermined | 23.6 |
Patient 23 | Saliva 23 | Not Detected | Not detected | Undetermined | Undetermined | Undetermined | 29.1 |
Patient 24 | Saliva 24 | Not Detected | Not detected | Undetermined | Undetermined | Undetermined | 25.2 |