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. 2021 Feb 23;10:e65146. doi: 10.7554/eLife.65146

Figure 1. BisMapR, an RNase H-based strand-specific native R-loop detection strategy.

Figure 1.

Schematic of the BisMapR protocol. R-loops are released from cells using MapR and subjected to non-denaturing bisulfite conversion. Bisulfite-converted products are directly processed for second-strand synthesis in the presence of RNase H and dUTP. Adaptors are ligated to resultant dsDNA. Treatment with uracil DNA glycosylase (UDG) degrades all uracil-containing DNA molecules. Remaining DNA is tagged with paired-end barcoded index primers, amplified, sequenced, and strand-specifically aligned. Created with BioRender.com.