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. 2021 Feb 5;12:597566. doi: 10.3389/fgene.2021.597566

FIGURE 2.

FIGURE 2

Tissue aging phenotypes in p16/p21-TKO mice. (A) The bone density measured by miroCT. Micro CT images of distal femoral metaphysis from mice with different genotypes. The femurs are shown from the coronal (upper panel), sagittal (middle panel), and transverse (lower panel) planes, respectively. Quantification of the bone density and statistical analysis. **p = 0.01,***p < 0.01. (B) HE staining of testes. The testes from p21-G3TKO and G5DKO mice were undergoing severe degeneration, showing a lack of spermatogenesis in seminiferous tubules. The deficiency of p16 in G5DKO rescued this testis degeneration phenotype. (C) HE staining of intestines. The intestines from p21-G3TKO and G5DKO mice were undergoing severe degeneration, showing an irregular intestinal villi structure, especially in crypt structures (10×, arrows pointed). At higher magnification, the crypts of p21-G3TKO mice seemed to be swollen and enlarged in size, while the crypts of G5TKO mice were empty due to loss of cells (40X, arrows pointed). p16 deficiency rescued the loss of crypt structure in G5DKO.