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. 2021 Feb 2;12:604435. doi: 10.3389/fphar.2021.604435

FIGURE 2.

FIGURE 2

Survival of C . elegans during exposure to the pathogenic bacterium P. luminescens.C. elegans was treated with seven different TCM plant preparations (30 μg/ml), and DMSO as control, starting at the L4 stage (A) Pathogenic stress due to P. luminescens exposure was initiated on the 3rd or 7th day of adulthood, and the survival during stress was monitored in the different treatment groups. The values represent the mean survival ± SEM from three biological replicates. Significant differences were determined by log-rank test and Bonferroni correction with *p < 0.05; **p < 0.01; ***p < 0.001 or ****p < 0.0001. In addition, survival curves are shown for nematodes treated with E . ulmoides and C . chinensis extracts, which were exposed to the pathogen starting on the 3rd (B) and 7th (C) day of adulthood (D) The growth of P. luminescens and (E) E . coli OP50 was monitored during extract exposure and the colony-forming units per time point are shown.