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. 2021 Feb 3;12:583453. doi: 10.3389/fphys.2021.583453

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Copyright © 2021 Repetti, Majumder, De Oliveira, Meth, Yangchen, Sharma, Srivastava and Rohatgi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical pERK staining in murine kidney of sham (A) and solitary kidney (B) and dual immunofluorescence staining of pERK (GREEN) and DBA (RED) in sham (C) and unilateral nephrectomy (D). The black arrows identify the cells staining for pERK in the kidneys of sham (A) and after nephrectomy (B). Based on the morphology of pERK expressing cells, most of the staining localizes to the distal nephron in the cortex. To identify the tubular segment staining for pERK, sham (C) and nephrectomy (D) kidney samples were stained with DBA, to identify principal cells, and anti-pERK antibody. The white arrows identify areas of co-localization of DBA and pERK fluorescent signal.