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. 2021 Feb 3;12:583453. doi: 10.3389/fphys.2021.583453

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Copyright © 2021 Repetti, Majumder, De Oliveira, Meth, Yangchen, Sharma, Srivastava and Rohatgi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluid shear stress (FSS) does not alter abundance of α- or γ-ENaC in whole cell protein lysate (A–C), but treatment with U0126 reduces γ-ENaC and raises α-ENaC expression (D–F). MpkCCD cells grown in plastic plates were either exposed to static (n = 8) conditions or FSS of 0.4 dynes/cm² for 24 h (n = 9) and then whole cell protein isolated. Immunoblotting (A) showed that γ- and α-ENaC subunit (B,C) expression was unaffected by FSS. On the other hand, steady state γ-ENaC protein expression was reduced in FSS and U0126 (n = 6; #, p < 0.05) exposed mpkCCD cells compared shear (n = 4) controls (D,E); however, α-ENaC expression increased in the same FSS and U0126 treated cells (D,F).