Table 1.
Proposed synthetic pathways of the principal fucosyloligosaccharides used to identify Secretor (Se) and Lewis (Le) phenotypes based on their presence/absence in the 10 milk samples.
| Starting structure | LNT | Lactose | LNT | LNnT | Lactose | 2'FL | ||
|---|---|---|---|---|---|---|---|---|
| Subject | HMO phenotype | Se+ | Le+ | Proposed HMO phenotype | ||||
| HMO | LNFP I | 2'FL | LNFP II | LNFP III | 3FL | LDFT | ||
| 1 | bld | bld | bld | bld | bld | bld | Se–Le– | |
| 2 | √ | √ | √ | √ | √ | √ | Se+Le+ | |
| 3 | bld | bld | √ | √ | √ | bld | Se–Le+ | |
| 4 | bld | bld | √ | √ | √ | bld | Se–Le+ | |
| 5 | bld | bld | bld | bld | bld | bld | Se–Le– | |
| 6 | bld | bld | √ | √ | √ | bld | Se–Le+ | |
| 7 | bld | bld | √ | √ | √ | bld | Se–Le+ | |
| 8 | bld | bld | bld | bld | bld | bld | Se–Le– | |
| 9 | √ | √ | bld | bld | bld | bld | Se+Le– | |
| 10 | √ | √ | √ | √ | √ | √ | Se+Le+ | |
LNFP I, lacto-N-fucopentose I; LNFP II, lacto-N-fucopentose II; LNFP III, lacto-N-fucopentose III; LNT, lacto-N-tetraose; LNnT, lacto-N-neotetraose; LDFT, lactodifucotetraose; 2'FL, 2'fucosyllactose; 3FL, 3-fucosyllactose; Se, secretor; Le, Lewis; √, Detected; bld, Below limit of detection. HMO concentrations below 20 μM were considered below the detection limit.