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. 2021 Feb 3;11:627007. doi: 10.3389/fgene.2020.627007

Figure 6.

Figure 6

linc-UR-B1 is expressed in meiotic and post-meiotic germ cells and positively correlates with USP18. (A) Measure of linc-UR-B1 levels by quantitative PCR (qPCR) in purified testicular cell populations (spermatocytes, spermatids, peritubular cells, and Leydig cells) and Sertoli cells. Results (± SEM) shown as expression (2-ΔCt) relative to ACTB. (B,D) Analysis of linc-UR-B1 (B) and USP18 (D) expression in testicular cell populations (clusters 1–13, three donors). (C,E) Analysis of linc-UR-B1 (C) and USP18 (E) expression in clusters of germ cells populations at different stages of prophase I (three donors). Note that clusters 14–18 are subclusters of clusters 3 and 4 studied in (B) and (D). (F,G) Positive correlation of linc-UR-B1 and USP18 expression in germ cell populations (F, clusters 1–8) and in germ cells at different stages of prophase I (G, clusters 14–18). All data were retrieved from the alignment of single cell RNA-seq reads provided by (Guo et al., 2018), filtered for unique reads, and expressed (± SEM) as percentage expression (normalized expression in one population vs. all populations analyzed). Cell populations expressing linc-UR-B1 are highlighted in red.