Figure 1.

Relative messenger RNA (mRNA) levels of AsKunitz, As8.9kDa, and AsBasicTail in Amblyomma sculptum instars during feeding. Measurements were performed using quantitative polymerase chain reaction in whole body for larvae (A–C) and nymphs (D–F) and in the salivary glands for females (G–I). Feeding = 3 days of feeding. Statistical analysis: (A–F)—t-test, (G–I)—ANOVA–Dunnett, *p < 0.05, **p < 0.01, and ***p < 0.001 indicate difference from the fasting group. Data is shown as mean ± standard deviation (SD).