Figure 2.

Intracellular DNA stimulates transcription of MHC-II and co-stimulatory molecules. (A) BMDMs or (B) HD11 cells were transfected with HT-DNA, CT-DNA, or Poly(I:C) and transcription of BLB1, BLB2, DMB1, DMB2, CD40, and CD86 measured by qRT-PCR 6 h later. (C) HD11 cells were stimulated with HT-DNA, CT-DNA, or Poly(I:C) and 6 h later phagocytosis was monitored by FITC-conjugated, zymosan coated bead uptake. Histograms of non-treated versus treated cells (left panels) and respective percentages of FITC positive cells for each treatment tested (right panel) are presented. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference. Data is representative of two or more replicates.