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. 2021 Feb 1;11:613079. doi: 10.3389/fimmu.2020.613079

Figure 3.

Figure 3

STING and TBK1 contribute to DNA-driven transcriptional responses in chicken BMDMs. (A) HD11 and (B) BMDM cells were treated with 2’3’cGAMP (10 μg/ml) and qRT-PCR carried out 6 h later for the indicated genes. (C) BMDM were treated with the STING inhibitor H-151 (10 uM) or TBK1 inhibitor BX795 (1 uM) for 1 h before transfection with HT-DNA and CT-DNA. Six hours later, RNA was extracted and qRT-PCR carried out for the indicated genes. (D) BMDM were treated with the STING inhibitor H-151 (10 uM) or TBK1 inhibitor BX795 (1 uM) for 1 h before treatment with 2’3’cGAMP (10 μg/ml). Six hours later, RNA was extracted and qRT-PCR carried out for the indicated genes. (E) HD11 cells were treated with 2’3’cGAMP (2.5 µg/ml) 6 h later phagocytosis was monitored by FITC-conjugated, zymosan coated bead uptake. *p < 0.05, **p < 0.01, ***p < 0.001; ****p < 0.0001; ns: no significant difference. Data is representative of two or more replicates.